无锡康纳生物科技有限公司

盐酸克仑特罗酶联免疫(ELISA)快速检测试剂盒1 概要盐酸克仑特罗（俗称瘦肉精）为一种人工合成的β兴奋剂，具有扩张支气管的作用，常用来防治哮喘、肺气肿等肺部疾病。当其应用剂量达到治疗量的5~10倍时，具有能量重分配作用，可使肌肉合成增加，脂肪沉积减少，因此俗称为“瘦肉精”。在畜牧业生产中一些非法使用者将其作为一种生长促进剂添加到动物饲料中，用以改善胴体的品质，同时也造成食品中盐酸克仑特罗（瘦肉精）的残留。当人们食用盐酸克仑特罗残留的产品后会出现一系列的中毒症状。我国禁止在畜牧业中使用盐酸克仑特罗。本试剂盒可用于定量、定性检测饲料、动物尿液以及动物组织等样本中盐酸克仑特罗的残留。2 原理本试剂盒采用直接竞争ELISA法检测样本中的盐酸克仑特罗。在微孔板上预包被抗盐酸克仑特罗单克隆抗体，样本或标准溶液中的盐酸克仑特罗和HRP标记的盐酸克仑特罗竞争结合微孔板中抗盐酸克仑特罗单克隆抗体，洗涤后用H₂O₂-TMB底物系统显色，样本吸光度值与其所含盐酸克仑特罗量成负相关，与标准曲线比较即可得出盐酸克仑特罗含量。3 试剂盒组份每个试剂盒中的试剂可进行96个测量（包括标准测定），试剂盒组份：                                                                                                                  包被有抗盐酸克仑特罗抗体微孔板····················································· 96孔盐酸克仑特罗标准溶液································································· 1.5 mL /瓶（6瓶）分别为0号：0 ppb ；1号：0.1 ppb ；2号：0.3 ppb ；3号：0.9 ppb ；4号：2.7 ppb ；5号：8.1 ppb 盐酸克仑特罗-HRP标记物······································································· 1瓶(6 mL)20×浓缩洗涤液························································································· 1瓶(50 mL)底物溶液A································································································· 1瓶(7 mL)底物溶液B·································································································· 1瓶(7 mL)终止液（含2 M的硫酸）········································································· 1瓶(6 mL)封板膜········································································································· 3张说明书········································································································· 1份